Antioxidant activity of Heterotheca inuloides extracts and of some of its metabolites

作者：Coballase Urrutia Elvia; Pedraza Chaverri Jose; Camacho Carranza Rafael; Cardenas Rodriguez Noemi; Huerta Gertrudis Bernardino; Noel Medina Campos Omar; Mendoza Cruz Myrna; Delgado Lamas Guillermo; Javier Espinosa Aguirre J

来源：Toxicology, 2010, 276(1): 41-48.

DOI：10.1016/j.tox.2010.06.013

• Heterotheca inuloides
• Scavenger activity
• Reactive oxygen species

Arnica (Heterotheca inuloides) is a widely used medicinal plant in Mexico; it has been recognized as anti-inflammatory, analgesic, cytotoxic, scavenger of superoxide anion and also as a preventive of lipid peroxidation. In vivo studies have demonstrated a hepatoprotective action of the methanolic extract of this plant as well as of quercetin, one of its main components, and the evidence obtained pointed out to an antioxidant mechanism. In this work, we focused on the free radical scavenging capacity of acetonic and methanolic extracts of H. inuloides in comparison with reference compounds. The two extracts were 2-12 times more effective (IC50, mu g/mL) than the reference compounds to cope with the following radicals or molecules tested: 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(center dot+)), 2,2-diphenyl-1-picrylhydrazyl (DPPH center dot), peroxynitrite (ONOO-), superoxide (O-2(center dot-)), singlet oxygen (O-1(2)), hypochlorous acid (HOG), hydrogen peroxide (H2O2). hydroxyl (OH center dot). Additionally, five secondary metabolites isolated from the methanolic extract displayed potent concentration-dependent antioxidant effects against reactive oxygen species produced in vitro (IC50 values in the range of 0.018-4.31 mg/mL). D-Chiro-inositol showed the higher antioxidant effect against O-2(center dot-), H2O2 and OH center dot while spinasterol and quercetin were the most active against O-1(2) and ONOO-, respectively. The antioxidant properties of the extracts and metabolites tested partially support the wide use of this plant in traditional medicine.

• 出版日期2010-9-30