Procalcitonine, Nitric oxide and C-Reactive Protein concentrations in rats with experimentally-induced infectious and noninfectious inflammation

作者：Ulutas Pinar Alkim^*; Kiral Funda; Ural Muruvvet

来源：Ankara Universitesi Veteriner Fakultesi Dergisi, 2015, 62(2): 81-85.

DOI：10.1501/vetfak_0000002662

• CRP
• inflammation
• NO
• PCT
• rat

The aim of this study is to determine the alterations in procalcitonine (PCT), nitric oxide (NO) and C-reactive protein (CRP) levels in infectious and noninfectious inflammation and to evaluate their potential in the differentiation of inflammation. Totally 70 female Wistar rats (Group 1: n=21; Group 2, n=21, Group 3: n=21; Day 0: n=7), weighing 250-300 grams, were used in this study. Before the applications, blood samples were collected in seven rats and the time is accepted as Day 0. Three groups were formed each containing 21 rats. Stapylococcus aureus were administered at a concentration of 10 (6) CFU in the infection group (Group 1), subcutaneously. Turbentine oil at a dose of 0.5 mg/kg were injected subcutaneously to twenty-one rats in the noninfectious inflammation group (Group 2). In the control group, 21 rats were injected with saline subcutaneously. Blood samples were collected from randomly selected 7 rats in each group on the 1st, 4th and the 7th days. PCT, NO, and CRP analyzes were performed with commercial test kits in the ELISA reader. According to the obtained results, we determined that serum PCT, NO and CRP levels increased after administrations. The rises of CRP and PCT concentrations were not statistically significant but it was higher in infectious group levels than the other two groups. Based on these results, it can be said that NO shows significant increases during infectious inflammation and can be used for diferential diagnostic purposes in clinics.

• 出版日期2015