A Rapid and Specific C-Reactive Protein Immunoassay Driven by an Electrophoresis System Based on Protein Enrichment in a 3D Filter

作者：Zhang, Fangfang; Ma, Junjie; Watanabe, Junji; Liu, Huiyu^*; Shen, Heyun^*

来源：Nanoscience and Nanotechnology Letters, 2017, 9(4): 425-432.

DOI：10.1166/nnl.2017.2328

• CRP
• ELISA
• Electrophoresis
• Polyelectrolyte Multilayer

A rapid, sensitive and simple electrophoresis immunoassay was developed using a positive polyelectrolyte multilayer (PEM)-modified cellulose acetate (CA) membrane filter (PEM-CA). C-reactive protein (CRP), a cardiac and inflammation marker, was used as a measurement model. This rapid immunoassay was carried out using electrophoresis to drive the antigen movement, thus allowing local condensation of CRP in the proximity of an antibody-enriched PEM-CA filter. This overcame the diffusion resistance that normally dictates the time required for conventional ELISA. Moreover, electrophoresis could enhance the CRP specificity in a mixed solution of serum proteins because each protein has a unique electrophoretic velocity. The calibration curve for electrophoresis detection had a wide linear range of concentration from 0.013 to 50 nM, with a limit of detection of 33 pM, regardless of the presence of other proteins. The incubation time for antigen-antibody reaction was only 2 min, or about 1/30th of the time required for a conventional ELISA system. We conclude that this electrophoresis detection system should be developed further as a rapid, precise, and simple system for various immunosensors, using PEM-modified membrane filters.

• 出版日期2017-4
• 单位北京化工大学