The sensitivity of Na+, K+ ATPase as an indicator of blood diseases

作者：Omar Abulnaja Kkalid; Ahmed Kherd Ali; Helmi Nawal Mohammed; Abdullah Kumosani Taha; Qarii Mohamad H; Hasan Huwait Etimad; Ashwag Albukhari; Nabil Alaama Mohammed; Abdu Al Ghamdi Maryam; Salama Moselhy Said^*

来源：African Health Sciences, 2017, 17(1): 262-269.

DOI：10.4314/ahs.v17i1.32

• Na+
• K+-ATPase
• red blood cell
• plasma membrane
• iron deficiency anemia
• thalassemia
• sickle cell anemia
• indicator

Background: Blood-related hereditary diseases are widespread in Eastern and SouthWestern regions of Saudi Arabia until recently. In this study, we used Na+, K+ ATPase as an enzymatic indicator for the diagnosis of the diseases.
Materials and methods: Individuals with different blood diseases (iron deficiency (n=13), anemia (n=14), thalassemia (n=16) and sickle cell anemia (n=12) were studied for Na+, K+-ATPase activity in the plasma membrane of red blood cell and compared with those of the healthy ones (n=20) of the same age and gender living in Jeddah, Saudi Arabia.
Results: There was a significant elevation in the specific activity of Na+, K+ ATPase in individuals with anemia compared with those of control (0.0094 + 0.001 nmol/mg protein/min versus 0.0061 +/- 0.001). On the other hand, there was a significant reduction in enzyme activity in thalassemia (0.0028 +/- 0.002 nmol/mg protein/min) and sickle cell anemia cases (0.0042 +/- 0.001 nmol/mg protein/min) compared to the control group. The cut off value for Na+, K+ ATPase activity is 0.005 mu mol Pi/min-showing 94% sensitivity and 93% specificity for the differentiation of blood abnormality.
Conclusion: It can be recommended that the activity of Na+, K+-ATPase can be used for the diagnosis of individuals with blood diseases/disorders.

• 出版日期2017