科研之友
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摘要
Development of a technically simple yet effective method for paraquat (PQ) detection is of great importance due to its high clinical and environmental relevance. In this study, we developed a pyranine-based fluorescent "turnoff" method for PQsensing based on pyranine-PQinteraction. We investigated the dependence of analytical performance of this method on the experimental conditions, such as the ion strength, medium pH, and so on. Under the optimized conditions, the method is sensitive and selective, and could be used for PQ detection in real-world sample. This study essentially provides a readily accessible fluorescent system for PQsensing which is cheap, robust, and technically simple, and it is envisaged to find more interesting clinical and environmental applications.
