Mutation of a raft-targeting signal in the transmembrane region retards transport of influenza virus hemagglutinin through the Golgi

作者：Engel Stephanie; de Vries Maren; Herrmann Andreas; Veit Michael^*

来源：FEBS Letters, 2012, 586(3): 277-282.

DOI：10.1016/j.febslet.2012.01.002

• Influenza virus
• Hemagglutinin
• Golgi
• Vesicular transport
• Transmembrane region
• Palmitoylation
• Raft

Inclusion of proteins into membrane-rafts favours interactions required for virus assembly but has also been proposed to facilitate vesicular transport of proteins. The hemagglutinin (HA) of influenza virus contains a raft-targeting sequence in the outer leaflet of its transmembrane region. We report that its mutation enhances co-localization of HA with a cis-Golgi marker and retards Golgi-localized processing, such as acquisition of Endo-H resistant carbohydrates and proteolytic cleavage. In contrast, trimerization of the molecule in the ER and transport to the apical membrane were not affected. The second signal for raft-targeting, S-acylation at cytoplasmic cysteines, did not retard HA transport.

• 出版日期2012-2-3