Development of a fast ELISA for the specific detection of both leucomalachite green and malachite green

作者：Jiang Yousheng^*; Chen Li; Hu Kun; Yu Wenjuan; Yang Xianle; Lu Liqun

来源：Journal of Ocean University of China, 2015, 14(2): 340-344.

DOI：10.1007/s11802-015-2407-5

• monoclonal antibody
• Malachite Green
• ELISA
• fish

Malachite green (MG), a dye, is an antifungal agent that has been used to treat and prevent fish diseases. It is metabolized into reduced leucomalachite green forms (LMG) that may reside in fish muscles for a long period, thus being harmful to human health. The aim of this study was to develop a competitive and direct enzyme-linked immunosorbent assay (ELISA) to detect MG and LMG specifically. The monoclonal antibody (mAb) to LMG was generated using a hybridoma technique. The obtained mAb showed good cross-reactivity (CR) to malachite green (MG), but not to crystal violet (CV) and Brilliant Green (BG). The mAb was used to develop a fast detecting ELISA of MG and LMG in fish. By introducing the conjugation LMG-HRP, the detection capability was 0.37 ng mL(-1) for MG and LMG. The mean recovery from spiked grass carp tissues ranged from 76.2% to 82.9% and the coefficients of variation varied between 1.8% and 7.5%. The stable and efficient monoclonal cell line obtained is a sustainable source of sensitive and specific antibody to MG and LMG.

• 出版日期2015-4
• 单位上海海洋大学