Novel Phage Group Infecting Lactobacillus delbrueckii subsp lactis, as Revealed by Genomic and Proteomic Analysis of Bacteriophage Ldl1

作者:Casey Eoghan; Mahony Jennifer; Neve Horst; Noben Jean Paul; Dal Bello Fabio; van Sinderen Douwe*
来源:Applied and Environmental Microbiology, 2015, 81(4): 1319-1326.
DOI:10.1128/AEM.03413-14

摘要

Ldl1 is a virulent phage infecting the dairy starter Lactobacillus delbrueckii subsp. lactis LdlS. Electron microscopy analysis revealed that this phage exhibits a large head and a long tail and bears little resemblance to other characterized phages infecting Lactobacillus delbrueckii. In vitro propagation of this phage revealed a latent period of 30 to 40 min and a burst size of 59.9 +/- 1.9 phage particles. Comparative genomic and proteomic analyses showed remarkable similarity between the genome of Ldl1 and that of Lactobacillus plantarum phage ATCC 8014-B2. The genomic and proteomic characteristics of Ldl1 demonstrate that this phage does not belong to any of the four previously recognized L. delbrueckii phage groups, necessitating the creation of a new group, called group e, thus adding to the knowledge on the diversity of phages targeting strains of this industrially important lactic acid bacterial species.

  • 出版日期2015-2