Hydrophilic Fluorescent Nanogel Thermometer for Intracellular Thermometry

作者:Gota Chie; Okabe Kohki; Funatsu Takashi; Harada Yoshie; Uchiyama Seiichi*
来源:Journal of the American Chemical Society, 2009, 131(8): 2766-+.
DOI:10.1021/ja807714j

摘要

The first methodology to measure intracellular temperature is described. A highly hydrophilic fluorescent nanogel thermometer developed for this purpose stays in the cytoplasm and emits stronger fluorescence at a higher temperature. Thus, intracellular temperature variations associated with biological processes can be monitored by this novel thermometer with a temperature resolution of better than 0.5 degrees C.