A comparison of the main structures of N-glycans of porcine islets with those from humans

作者：Miyagawa Shuji^*; Maeda Akira; Kawamura Takuji; Ueno Takehisa; Usui Noriaki; Kondo Sachiko; Matsumoto Shinichi; Okitsu Teru; Goto Masafumi; Nagashima Hiroshi

来源：Glycobiology, 2014, 24(2): 125-138.

DOI：10.1093/glycob/cwt088

• N-glycan
• pig islets
• sulfated glycan
• xenotransplantation

After producing alpha 1-3-galactosyltransferase knockout (GKO) pigs, most of the organs of these pigs showed less antigenicity to the human body. However, wild-type adult pig islets (API) that originally contained negligible levels of alpha-galactosidase now showed a clear antigenicity to human serum. In this study, N-glycans were isolated from both APIs and human islets. Their structures were then analyzed by a mapping technique based on their high-performance liquid chromatography elution positions and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometric data. Both preparations contained substantial amounts of high-mannose structures. The N-glycans from human islets were separated into 17 neutral, 8 mono-sialyl and 4 di-sialyl glycans, and the API glycans were comprised of 11 neutral, 8 mono-sialyl, 3 di-sialyl, 2 mono-sulfated, 3 mono-sialyl-mono-sulfated and 1 di-sulfated glycans. Among them, the API preparation contained one neutral, five mono-sialyl glycans and six sulfated glycans that were not detected in human islets. The structures of 9 of these 12 could be clearly determined. In addition, a study of the sulfate-depleted API suggests that sulfate residues could be antigenic to humans. The data herein will be helpful for future studies of the antigenicity associated with API.

• 出版日期2014-2