A tandem-repeat galectin involved in innate immune response of the pearl oyster Pinctada fucata

作者：Wang, Zhongliang; Jian, Jichang; Lu, Yishan; Wang, Bei; Wu, Zaohe^*

来源：Marine Genomics, 2011, 4(3): 229-236.

DOI：10.1016/j.margen.2011.06.004

• SSH
• EST
• RACE
• Galectin
• Bacterial challenge
• Real-time PCR

The cDNA of a tandem-repeat galectin from the pearl oyster Pinctada fucata (designated PfGal) was cloned by expressed sequence tag (EST) and rapid amplification of cDNA ends (RACE) techniques. The full-length cDNA of PfGal was 1386 bp, consisting of a 5' untranslated region (UTR) of 26 bp, a 3' UTR of 313 bp, and an open reading frame (ORF) of 1047 bp encoding a polypeptide of 348 amino acids with a predicted molecular weight of 38.09 kDa and theoretical isoelectric point of 8.49. Similar to other tandem-repeat galectins, PfGal contained two tandem carbohydrate recognition domains (CRDs), with typical conserved motifs which were important for carbohydrate recognition, and it appeared to possess neither a signal peptide nor a transmembrane domain. Fluorescent quantitative real-time PCR analyses indicated that PfGal mRNA was highly expressed in hemocytes, digestive gland and mantle, and its expression was increased in all studied tissues after Vibrio alginolyticus challenge. The temporal expression of PfGal mRNA in hemocytes challenged by V. alginolyticus was clearly time-dependent and reached the maximum level at 6 h post-challenge, and then recovered to the original level. These results collectively indicated that PfGal may be involved in the immune response against bacterial infection and clearance of bacterial pathogens in P. fucata.

• 出版日期2011-9
• 单位广东海洋大学