Design and synthesis of a novel lanthanide fluorescent probe (EuIII-dtpa-(bis)melamine) and application in melamine detection in milk products

作者：Jiang, Xiaoqing; Tian, Fengyun; Yang, Fan; Dou, Xuekai; Wang, Jun^*; Song, Youtao^*

来源：Sensors and Actuators B: Chemical , 2017, 238: 605-612.

DOI：10.1016/j.snb.2016.07.101

• Fluorescence probe
• Aminopolycarboxylic acid ligands
• Eu(III) complexes
• Fluorescence spectroscopy
• Melamine

Eu-III-dtpa-bis(melamine) was designed and synthesized as a novel targeting fluorescence probe for melamine detection in milk products. The ligand field change around Eu-III caused by melamine leads to a highly selective fluorescence "turn-on" response. The competitive assay showed that the presence of other analogues (cyanuric acid, cyanuric chloride, trichloroisocyanuric acid and acetoguanamine) rarely affected the turn-on fluorescence intensity in probing melamine. The obtained results displayed a linear correlation between melamine concentration and fluorescence intensity. The linear equation was y = 38.68x + 568.32 (R-2 = 0.9972) with melamine concentration ranged from 5 to 100 mu mol/L. When melamine concentration reaches 100 mu mol/L, the fluorescence intensity becomes the maximum. The formed Eu-III-dtpa-bis(melamine)-melamine complex was extremly stable with the stability constant (k) of 9.90 x 10(6) and the complexation ratio was 1:1. The detection limit (LOD) was 0.3743 mu mol/L. The recoveries ranged from 99.80 to 100.50%, when melamine was detected in some milk products. It can be confirmed that Eu-III-dtpa-bis(melamine) shows high selectivity, sensitivity, targeting and recognition ability in melamine detection.

• 出版日期2017-2
• 单位辽宁大学