Acetyl-L-carnitine partially prevents benzene-induced hematotoxicity and oxidative stress in C3H/He mice

作者：Sun, Rongli; Zhang, Juan^*; Wei, Haiyan; Meng, Xing; Ding, Qin; Sun, Fengxia; Cao, Meng; Yin, Lihong; Pu, Yuepu^*

来源：Environmental Toxicology and Pharmacology, 2017, 51: 108-113.

DOI：10.1016/j.etap.2017.02.013

• Benzene
• Acetyl-L-carnitine
• Hematotoxicity
• Mitochondrial dysfunction
• Oxidative stress

Benzene is an environmental pollutant and occupational toxicant which induces hematotoxicity. Our previous metabonomics study suggested that acetyl-L-carnitine (ALCAR) decreased in the mouse plasma and bone marrow (BM) cells due to benzene exposure. In the present study, the topic on whether ALCAR influences hematotoxicity caused by benzene exposure was explored. Thirty-two male C3H/He mice were divided into four groups: control group (C: vehicle, oil), benzene group (150 mg/kg body weight (b.w.) benzene), benzene + A1 group (150 mg/kg b.w. benzene + 100 mg/kg b.w. ALCAR), and benzene + A2 group (150 mg/kg b.w. benzene + 200 mg/kg b.w. ALCAR). Benzene was injected subcutaneously, and ALCAR was orally administrated via gavage once daily for 4 weeks consecutively. After the experimental period, the blood routine, BM cell number and frequency of hematopoietic stem/progenitor cell (HS/PC) were assessed. The mitochondrial membrane potential and ATP level were determined to evaluate the mitochondrial function. Reactive oxygen species (ROS), hydrogen peroxide (H2O2) and malondialdehyde (MDA) levels were also examined, and the comet assay was performed to measure oxidative stress. Results showed that ALCAR intervention can partially reduce the benzene-induced damage on BM and HS/PCs and can simultaneously alleviate the DNA damage by reducing benzene-induced H2O2, ROS, and MDA.

• 出版日期2017-4
• 单位东南大学