Adsorption of immunoglobulin Y in supermacroporous continuous cryogel with immobilized Cu2+ ions

作者：da Silva Junior Willer Ferreira; Cano Rodrigo; Totola Antonio Helvecio; de Carvalho Lorendane Milena; Cerri Marcel Otavio; dos Reis Coimbra Jane Sella; Pinto de Carvalho Gleidson Giordano; Aparecida de Carvalho Bruna Mara

来源：Journal of Chromatography A, 2015, 1395: 16-22.

DOI：10.1016/j.chroma.2015.03.052

• Immunoglobulin Y
• Adsorption isotherms
• Thermodynamic parameters
• Cryogel
• IMAC

The influence of temperature, pH and ionic strength on the adsorption of Immunoglobulin Y (IgY) in IDA-Cu2+ cryogel system was studied by batch equilibrium Measurements. The adsorptive equilibrium data were obtained at 17 and 27 degrees C, pH 5.0 and 6.5, and at ionic strength of 50 and 200 mmol L-1 NaCl. Langmuir, Freundlich and Langmuir-Freundlich models were fitted to equilibrium data, while the enthalpy of adsorption of IgY in IDA-Cu2+ cryogel system was calculated through Van't Hoff analysis. The binding of IgY on cryogel was stronger at 27 degrees C and lowest pH and ionic strength values, with apparent maximum adsorption capacity of 27 mg g(-1). The adsorption of protein in the resin was spontaneous in all analyzed cases. The results provide valuable information to enable the improvement of IgY purification processes.

• 出版日期2015-5-22