Identification of active transposon dTok, a member of the hAT family, in rice

作者：Moon Sunok; Jung Ki Hong; Lee Do Eun; Jiang Wen Zhu; Koh Hee Jong; Heu Mun Hue; Lee Dong Sun; Suh Hak Soo; An Gynheung^*

来源：Plant and Cell Physiology, 2006, 47(11): 1473-1483.

DOI：10.1093/pcp/pcl012

• hAT superfamily
• floral organ number 1/multiple pistil 2
• Oryza
• rice
• transposon

Recent completion of the sequencing of the rice genome has revealed that it contains > 40% repetitive sequences, most of which are related to inactive transposable elements. During the molecular analysis of the floral organ number1/multiple pistil 2 (fon1/mp2) mutant, we identified an active transposable element dTok0 that was inserted at the kinase domain of FON1, a homolog of CLAVATA1. Insertion of the element into FON1 generated an 8 bp duplication of its target sites, which is one of the major characteristics of the hAT family of transposons. The dTok0 element was actively transposed out of the FON1 gene, leaving 5-8 bp footprints. Reinsertion into a new location was observed at a low frequency. Analysis of the genome sequence showed that the rice cultivar 'Nipponbare' contains 25 copies of dTok elements; similar numbers were present in all the Oryza species examined. Because dTok0 does not encode a transposase, enzyme activity should be provided in trans. We identified a putative autonomous transposon, Tok1 that contains an intact open reading frame of the Ac-like transposase.

• 出版日期2006-11