An unusual beta-ketoacyl : acyl carrier protein synthase and acyltransferase motifs in TaK, a putative protein required for biosynthesis of the antibiotic TA in Myxococcus xanthus

作者：Paitan Y; Orr E; Ron EZ^*; Rosenberg E

来源：FEMS Microbiology Letters, 2001, 203(2): 191-197.

DOI：10.1111/j.1574-6968.2001.tb10840.x

• antibiotic biosynthesis
• polyketides post-modification
• polyketide synthase
• beta-ketoacyl synthase
• beta-ketoacyl : ACP synthase
• Myxococcus xanthus

The antibiotic TA of Myxococcus xanthus is produced by a type-1 polyketide synthase mechanism. Previous Studies have indicated that TA genes are clustered within a 36-kb region. The chemical structure of TA indicates the need for several post-modification steps. which are introduced to form the final bioactive molecule. These include three C-methylations. an O-methylation and a specific hydroxylation, In this study, we describe the genetic analysis of taK, encoding a specific polyketide beta -ketoacyl:acyl carrier protein synthase, which contains an unusual beta -ketoacyl synthase and acyltransferase motifs and is likely to be involved in antibiotic TA post-modification. Functional analysis of this beta -ketoacyl:acyl carrier protein synthase by specific gene disruption suggests that it is essential for the production of an active TA molecule.

• 出版日期2001-9-25