A comparison of glucose concentration in paired specimens collected in serum separator and fluoride/potassium oxalate blood collection tubes under survey %26apos;field%26apos; conditions

作者：Fernandez Lois; Jee Penny; Klein Mari Jill; Fischer Peter; Perkins Sherry L; Brooks Stephen P J^*

来源：Clinical Biochemistry, 2013, 46(4-5): 285-288.

DOI：10.1016/j.clinbiochem.2012.11.027

• Blood glucose
• Serum-separator
• Fluoride oxalate

Objectives: There are no direct comparisons of blood glucose values in samples collected with barrier serum tubes (SST (TM)) and NaF/potassium oxalate (NaF/KOx) plasma tubes. Collection of samples in SST (TM) tubes can offer considerable savings and specimen processing advantages for national level surveys. %26lt;br%26gt;Design and methods: Serum and plasma samples were collected under %26apos;field conditions%26apos; from a single draw of 3692 individuals participating in the Canadian Health Measures Survey. The samples were analyzed retrospectively using the VITROS GLU Slide method (glucose oxidase-based). %26lt;br%26gt;Results: There was a high rate of hemolysis in the NaF/KOx tubes (86.2%) while hemolysis was infrequently observed with the SST (TM) tubes (2%). Comparing only blood draws where no hemolysis was observed in both tubes (n=495; paired t-test) showed no effect of tube type on serum/plasma glucose concentrations. This was also observed when data was restricted to cases when only SST (TM) samples were not hemolyzed (n=3546; paired t-test). %26lt;br%26gt;Conclusions: These data show that both collection tubes can be used under survey collection and processing conditions to measure glucose with our assay system with no difference in reported results.

• 出版日期2013-3