NHS-mediated QDs-peptide/protein conjugation and its application for cell labeling

作者：Shan, Yaming; Wang, Liping^*; Shi, Yuhua; Zhang, Hao; Li, Hongmei; Liu, Hanzhi; Yang, Bai; Li, Tianyu; Fang, Xuexun; Li, Wei

来源：Talanta, 2008, 75(4): 1008-1014.

DOI：10.1016/j.talanta.2007.12.040

• quantum dot
• NHS-mediated conjugation
• cell labeling
• nuclear targeting signal

3-Mercaptopropyl acid-stabilized CdTe nanoparticles synthesized in aqueous solution are found to be able to conjugate with peptides or proteins mediated by N-hydroxysulfo-succinimide (NHS) but 1-ethyl-3(3-dimethylaminopropyl) carbodiimides hydrochloride (EDC). The reaction time and pH have been optimized. Gel-permeation HPLC was applied following the conjugation, which could quickly and simultaneously detect and purify the quantum dots (QDs) conjugates. The biological activities of QDs conjugates are maintained and give superior results in cell labeling. These results are encouraging regarding the application of QDs molecules for use in living cells, diagnostics and drug delivery.

• 出版日期2008-5-30
• 单位吉林大学