Application of real-time PCR in Ghana, a Buruli ulcer-endemic country, confirms the presence of Mycobacterium ulcerans in the environment

作者：Vandelannoote Koen^*; Durnez Lies; Amissah Diana; Gryseels Sophie; Dodoo Alfred; Yeboah Shirley; Addo Phyllis; Eddyani Miriam; Leirs Herwig; Ablordey Anthony; Portaels Francoise

来源：FEMS Microbiology Letters, 2010, 304(2): 191-194.

DOI：10.1111/j.1574-6968.2010.01902.x

• Mycobacterium ulcerans
• Buruli ulcer
• real-time PCR
• Ashanti region
• environmental samples
• small mammals

This study reports the first successful application of real-time PCR for the detection of Mycobacterium ulcerans, the causative agent of Buruli ulcer (BU), in Ghana, a BU-endemic country. Environmental samples and organs of small mammals were analyzed. The real-time PCR assays confirmed the presence of M. ulcerans in a water sample collected in a BU-endemic village in the Ashanti Region.

• 出版日期2010-3
• 单位河北医科大学