Iterative plug-and-play methodology for constructing and modifying synthetic gene networks

作者：Litcofsky Kevin D; Afeyan Raffi B; Krom Russell J; Khalil Ahmad S^*; Collins James J

来源：Nature Methods, 2012, 9(11): 1077-+.

DOI：10.1038/NMETH.2205

We present a methodology for the design, construction and modification of synthetic gene networks. This method emphasizes post-assembly modification of constructs based on network behavior, thus facilitating iterative design strategies and rapid tuning and repurposing of gene networks. The ease of post-construction modification afforded by this approach and the ever-increasing repository of components within the framework will help accelerate the development of functional genetic circuits for synthetic biology.

• 出版日期2012-11