Anti-inflammatory effects of Panax notoginseng saponins ameliorate acute lung injury induced by oleic acid and lipopolysaccharide in rats

作者：Chen, Yu Qing; Rong, Ling; Qiao, Jian Ou^*

来源：Molecular Medicine Reports, 2014, 10(3): 1400-1408.

DOI：10.3892/mmr.2014.2328

• acute lung injury
• alveolar liquid clearance
• epithelial sodium channel
• extravascular lung water
• Panax notoginseng saponins

This study investigated the effect of Panax notoginseng saponins (PNS) on acute lung injury (ALI) induced by oleic acid (OA) and lipopolysaccharide (LPS). A total of 28 Wistar rats were divided into four groups: sham; sham PNS; OA-LPS-induced ALI and ALI PNS. Lung tissue histology, lung wet-to-dry (W/D) weight ratio, extravascular lung water (EVLW) and epithelial sodium channel alpha (alpha ENaC) mRNA and protein expression were examined: In addition, levels of inflammatory cytokines, including tumor necrosis factor alpha (TNF-alpha), interleukin (IL)-6 and IL-10, as well as total leukocyte and neutrophil counts, were analyzed in rat bronchoalveolar lavage fluid (BALF) and serum. ALI PNS rats were observed to exhibit significantly lower pulmonary parenchymal damage and EVLW compared with ALI rats. Furthermore, total leukocyte and neutrophil counts, and levels of inflammatory cytokines were significantly decreased following PNS administration in ALI rats. In addition, the decrease in alpha ENaC mRNA and protein expression observed in the lung tissue of ALI rats was partially restored following PNS treatment. PNS treatment was demonstrated to ameliorate OA-LPS-induced ALI, potentially through restoration of alpha ENaC mRNA and protein expression and through PNS-induced anti-inflammatory effects.

• 出版日期2014-9
• 单位上海交通大学