A neural stem/precursor cell monolayer for neural tissue engineering

作者：Li Yi Chen; Tsai Li Kai; Wang Jyh Horng; Young Tai Horng^*

来源：Biomaterials, 2014, 35(4): 1192-1204.

DOI：10.1016/j.biomaterials.2013.10.066

• Neural stem/precursor cells (NSPCs)
• Differentiation
• Monolayer
• Epidermal growth factor (EGF)
• Fibronectin

The purpose of this study was to prepare a monolayer of neural stem/precursor cells (NSPCs) for neural tissue engineering applications. Two components present in serum, fibronectin and epidermal growth factor (EGF) were added into DMEM/F12 medium (termed medium B) to examine the effect of the migration-, proliferation- and differentiation-promoting potential on the cultured NSPCs, isolated from embryonic rat cerebral cortex. Compared with the serum effect, medium B also permitted neurosphere attachment onto the substrate surface and cell migration out of neurospheres extensively, but enhanced more extensive cell division and slowed down NSPC differentiation to generate a confluent NSPC monolayer. It was found the medium B-treated NSPCs possessed the capability to form typical neurospheres or to undergo differentiation into neuron-related cell types on various biomaterial surfaces. Therefore, we proposed a two-stage process for wound healing or nerve conduit preparation. Extensive NSPC division and MAP2-positive neuron differentiation were manipulated in NSPCs cultured in the medium B followed by the neuronal differentiation-favorable medium. These results should be useful for controlling the proliferation and differentiation of NSPCs on various biomaterials and conduits in neuroscience research.

• 出版日期2014-1