Age- and Sex-Associated Differences in the Glycopatterns of Human Salivary Glycoproteins and Their Roles against Influenza A Virus

作者：Qin, Yannan; Zhong, Yaogang; Zhu, Minzhi; Dang, Liuyi; Yu, Hanjie; Chen, Zhuo; Chen, Wentian; Wang, Xiurong; Zhang, Hua; Li, Zheng^*

来源：Journal of Proteome Research, 2013, 12(6): 2742-2754.

DOI：10.1021/pr400096w

• saliva
• age
• sex
• glycopattern
• lectin microarrays
• saliva microarrays
• influenza A virus

Recent studies have elucidated that expression of certain glycoproteins in human saliva is increased or decreased according to age; meanwhile, human saliva may inhibit viral infection and prevent viral transmission. However, little is known about the age- and sex-associated differences in the glycopatterns of human salivary glycoproteins and their significant roles against influenza A virus (IVA). Here, we investigate the glycopatterns of human salivary glycoproteins with 180 healthy saliva samples divided into six age/sex groups using lectin microarrays and fabricate saliva microarrays to validate the terminal carbohydrate moieties of glycoproteins in individual saliva samples. Furthermore, we assess the inhibiting and neutralizing activity of saliva against two strains of influenza A (H9N2) virus. We find that seven lectins (e.g., MAL-II and SNA) show significant age differences in both females and males, and seven lectins (e.g., WFA and STL) show significant sex differences in children, adults and elderly people. Interestingly, we observe that elderly individuals have strongest resistance to IVA partly by presenting more terminal alpha 2-3/6-linked sialic acid residues in their saliva, which bind with the influenza viral hemagglutinations. We conclude that age- and sex-associated differences in the glycopatterns of human salivary glycoproteins may provide pivotal information to help understand some age related diseases and physiological phenomena.

• 出版日期2013-6
• 单位中国农业科学院; 中国农业科学院哈尔滨兽医研究所; 陕西省人民医院; 西北大学