Aptamer-Based Fluorescent Switch for Sensitive Detection of Oxytetracycline

作者：Babaei Mahsa; Jalalian Seyed Hamid; Bakhtiari Hadi; Ramezani Mohammad; Abnous Khalil^*; Taghdisi Seyed Mohammad^*

来源：Australian Journal of Chemistry, 2017, 70(6): 718-723.

DOI：10.1071/CH16562

Oxytetracycline (OTC) is one of the most used antibiotics in veterinary medicine. There is a large concern about developing antibiotic resistance in humans as a result of the consumption of products contaminated with OTC, so a fast detection technique for an on-field screening test is highly in demand. Here we introduce a novel aptasensor for fast detection of OTC, based on a triple helix molecular switch (THMS) complex formation. The limit of detection (LOD) of this sensor was 1.67 and 6.44nM in phosphate buffer and milk samples, respectively. Moreover, the sensor showed a high selectivity towards OTC.

• 出版日期2017