NEOPLASTIC TRANSFORMATION AND CHARACTERIZATION OF HUMAN FIBROBLASTS BY TREATMENT WITH CO-60 GAMMA-RAYS AND THE HUMAN C-HA-RAS ONCOGENE

作者：JAHAN I; MIHARA K; BAI L; NAMBA M

来源：In Vitro Cellular & Developmental Biology-Animal, 1993, 29A(10): 763-767.

• HUMAN FIBROBLASTS
• NEOPLASTIC TRANSFORMATION
• C-HA-RAS

Human fibroblasts (KMST-6) immortalized by treatment with Co-60 gamma rays were further neoplastically transformed by transfection of the c-Ha-ras oncogene from human lung cancer. The ras-transfected cells formed undifferentiated fibrosarcoma in nude mice. One of the tumors was recultured and a neoplastic human fibroblast line, KMST-6/RAS, was established. To analyze multistep carcinogenesis of human cells, the cellular characteristics of these genetically matched immortalized (KMST-6) and neoplastic (KMST-6/RAS) cell lines were studied in detail. KMST-6/RAS cells showed an increased saturation density, colony formation on confluent monolayers of normal human fibroblasts, proliferation in neomycin-containing medium, anchorage-independent growth, and enhanced expression of the transfected c-Ha-ras oncogene, whereas the immortalized cells did not demonstrate these characteristics. Unexpectedly, growth of KMST-6/RAS cells was serum-dependent, although they were neoplastic. Interestingly, the neoplastic cells did not show the criss-crossing or piling up growth pattern characteristic of transformed rodent fibroblasts.

• 出版日期1993-10