MUC-1(-)/ESA(+) progenitor cells in normal, benign and malignant human breast epithelial cells

作者：Lue Xinquan; Li Huixiang^*; Xu Kejia; Nesland Jahn M; Suo Zhenhe

来源：Histology and Histopathology, 2009, 24(11): 1381-1390.

• Human mammary gland
• Stem/progenitor cell
• Immunohistochemistry

The existence of mammary epithelial stem/progenitor cells has been demonstrated in MUC-1(-)/ESA(+) subpopulations of breast epithelial cells. However, knowledge about the expression and localization in benign and malignant breast lesions is unknown. Using a double-staining immunohistochemistry method, we investigated MUC-1(-)/ESA(+) cells in 10 normal breast tissues, 49 cases with fibrocystic disease, 40 fibroadenomas, 36 invasive ductal carcinomas and the breast cancer cell lines MCF-7 and MDA-MB-468. In normal breast tissues MUC-1(-)/ESA(+) cells were mainly found in the suprabasal layer, but under the apical surface of the duct/alveolus. In the hyperplastic areas of fibrocystic disease, the number of this subpopulation of cells was higher than that in hypoplastic areas and in fibroadenomas. In invasive ductal carcinoma, the MMUC-1(-)/ESA(+) cells were heterogeneously present in different carcinoma nests. In the MCF-7 cell line most cells were MUC-1(-)/ESA(+), and in the MDA-MB-468 cell line MUC-1(-)/ESA(+) cells and MUC-1(-)/ESA(+) cells were almost equal. Our results show that the MUC-1(-)/ESA(+) subpopulation increases in fibrocystic disease within the hyperplastic areas, and varies in benign and malignant breast tumours, indicating that breast carcinogenesis may develop from malignant changes of normal MUC-1(-)/ESA(+) cells.

• 出版日期2009-11
• 单位郑州大学