High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases

作者：Chen Fuqiang; Pruett Miller Shondra M; Huang Yuping; Gjoka Monika; Duda Katarzyna; Taunton Jack; Collingwood Trevor N; Frodin Morten; Davis Gregory D^*

来源：Nature Methods, 2011, 8(9): 753-U96.

DOI：10.1038/nmeth.1653

Zinc-finger nucleases (ZFNs) have enabled highly efficient gene targeting in multiple cell types and organisms. Here we describe methods for using simple ssDNA oligonucleotides in tandem with ZFNs to efficiently produce human cell lines with three distinct genetic outcomes: (i) targeted point mutation, (ii) targeted genomic deletion of up to 100 kb and (iii) targeted insertion of small genetic elements concomitant with large genomic deletions.

• 出版日期2011-9