Mutating a conserved cysteine in GPIHBP1 reduces amounts of GPIHBP1 in capillaries and abolishes LPL binding

作者：Allan Christopher M; Jung Cris J; Larsson Mikael; Heizer Patrick J; Tu Yiping; Sandoval Norma P; Dang Tiffany Ly P; Jung Rachel S; Beigneux Anne P^*; de Jong Pieter J; Fong Loren G^*; Young Stephen G^*

来源：The Journal of Lipid Research, 2017, 58(7): 1453-1461.

DOI：10.1194/jlr.M076943

• chylomicrons
• endothelial cells
• lipids/chemistry
• lipolysis and fatty acid metabolism
• triglycerides
• glycosylphosphatidylinositol-anchored HDL binding protein 1
• lipoprotein lipase

Mutation of conserved cysteines in proteins of the Ly6 family cause human disease-chylomicronemia in the case of glycosylphosphatidylinositol-anchored HDL binding protein 1 (GPIHBP1) and paroxysmal nocturnal hemo-globinuria in the case of CD59. A mutation in a conserved cysteine in CD59 prevented the protein from reaching the surface of blood cells. In contrast, mutation of conserved cysteines in human GPIHBP1 had little effect on GPIHBP1 trafficking to the surface of cultured CHO cells. The latter findings were somewhat surprising and raised questions about whether CHO cell studies accurately model the fate of mutant GPIHBP1 proteins in vivo. To explore this concern, we created mice harboring a GPIHBP1 cysteine mutation (p. C63Y). The p. C63Y mutation abolished the ability of mouse GPIHBP1 to bind LPL, resulting in severe chylomicronemia. The mutant GPIHBP1 was detectable by immunohistochemistry on the surface of endothelial cells, but the level of expression was. similar to 70% lower than in WT mice. The mutant GPIHBP1 protein in mouse tissues was predominantly monomeric. We conclude that mutation of a conserved cysteine in GPIHBP1 abolishes the ability of GPIHBP1 to bind LPL, resulting in mislocalization of LPL and severe chylomicronemia. The mutation reduced but did not eliminate GPIHBP1 on the surface of endothelial cells in vivo.-Allan, C. M., C. J. Jung, M. Larsson, P. J. Heizer, Y. Tu, N. P. Sandoval, T. L. P. Dang, R. S. Jung, A. P. Beigneux, P. J. de Jong, L. G. Fong, and S. G. Young. Mutating a conserved cysteine in GPIHBP1 reduces amounts of GPIHBP1 in capillaries and abolishes LPL binding.

• 出版日期2017-7