Development of an indirect immunofluorescence technique for the evaluation of generated antibody titers against Erysipelothrix rhusiopathiae in captive bottlenose dolphins (Tursiops truncatus)

作者：Jose Bernal Guadarrama Maria; Garcia Parraga Daniel; Fernandez Gallardo Nuhacet; Zamora Padron Rafael; Pacheco Victor; Reyes Batlle Maria; Valladares Basilio; Lorenzo Morales Jacob^*; Martinez Carretero Enrique

来源：Archives of Microbiology, 2014, 196(11): 785-790.

DOI：10.1007/s00203-014-1020-0

• Erysipelothrix rhusiopathiae
• Bottlenose dolphin
• Diagnosis
• Vaccination
• IIF

Erysipelothrix rhusiopathiae is the causative agent of erysipelas, a disease of many mammalian and avian species, mainly swine and turkeys. In cetaceans, erysipelas is considered to be the most common infection in juvenile individuals, which have not been vaccinated. Moreover, the disease manifest in both forms, the dermatologic and the acute septicemic forms, has been reported in various species of dolphins and whales. It is difficult to diagnose erysipelas by currently available approaches. Moreover, it is mainly based on culture methods and also PCR methods, which are currently being developed. At the present stage, prophylactic approaches are based on antibiotic therapy and vaccination mostly with porcine erysipelas vaccines. In the present study, an Indirect Immuno Fluorescence method for the detection of dolphin antibodies levels against E. rhusiopathiae was developed and applied in two different groups of captive bottlenose dolphins (Tursiops truncatus) from Loro Parque (Tenerife, Canary Islands, Spain) and L%26apos;OceanogrA fic de Valencia (Valencia, Spain) in order to check the tittering levels of antibodies after application of porcine erysipelas vaccines in the studied dolphins.

• 出版日期2014-11