Application of a high-throughput, parallel HPLC system for quantitative chiral analysis of pantoprazole

作者：Wang, Hao; Sun, Yantong; Yang, Yan; Guo, Wei; Zheng, Mi; Zhang, Weiping; Fawcett, J. Paul; Gu, Jingkai^*

来源：Bioanalysis, 2015, 7(23): 2981-2990.

DOI：10.4155/bio.15.215

• LIQUID-CHROMATOGRAPHY SYSTEM
• PHARMACOKINETICS
• CYP2C19
• VALIDATION

Background: Chromatographic separation of enantiomers is considered a task in analytical chemistry particularly for high sample throughput. This paper describes a high-throughput parallel HPLC-MS/MS method for the determination of pantoprazole enantiomers. Results: Baseline separation of pantoprazole enantiomers was achieved on a Chiralcel OZ-RH column in a run time of 4.5 min. Assays for enantiomers were linear with satisfactory intra- and inter-day precision and accuracy. The assay was suitable for high-throughput analysis as shown by its successful application to a chiral PK study in beagle dog. Conclusion: A high-throughput parallel HPLC-MS/MS assay for pantoprazole has been developed and validated. This method provides nearly twofold increased sample throughput, and was shown to be suitable for application in PK studies.

• 出版日期2015
• 单位吉林大学