Nitrosomonas sp Based biosensor for ammonium nitrogen measurement in wastewater

作者：Raud Merlin^*; Lember Erki; Jogi Eerik; Kikas Timo

来源：Biotechnology and Bioprocess Engineering, 2013, 18(5): 1016-1021.

DOI：10.1007/s12257-013-0078-x

• ammonia nitrogen
• microbial biosensor
• AOB
• Nitrosomonas sp.
• wastewater analysis

A bacterial culture of Nitrosomonas sp. was isolated from a nitrifying biofilm to construct a biosensor for ammonium nitrogen (NH (4) (+) -N) measurements in high ammonia wastewaters. The pure culture of microorganisms was immobilized into agarose gel matrix to attain a stable biosensor with a long service life. Biosensors were calibrated using (NH4)(2)SO4 solution and a steady-state method. Subsequently, several experiments with synthetic and industrial wastewaters were conducted. A linear range up to 20 mg/L of NH (4) (+) -N, and sensitivities between 0.030 and 0.036 were gained with biosensors. During 14 days of stable service life of the Nitrosomonas sp. biosensors, variation of the signal was less than 7%. Response times of biosensors were 15 similar to 25 min, while recovery times were up to 25 min. Measurements with high ammonia content synthetic and industrial wastewaters were conducted, and 8.3 and 5.6% over estimation of NH (4) (+) -N was gained, respectively, compared with results of Nessler method. In spite of the small overestimation, the biosensor based on a pure culture of Nitrosomonas sp. and calibrated with (NH4)(2)SO4 is suitable for the analysis of NH (4) (+) -N in high ammonia content wastewaters.

• 出版日期2013-9