Development of a procedure to simultaneously isolate RNA, DNA, and proteins from characterizing cells invading or cultured on chitosan scaffolds

作者：Tchemtchoua Victor T; Atanasova Ganka; Aqil Abdelhafid; Maquet Veronique; Jerome Christine; Poumay Yves; Colige Alain^*

来源：Analytical Biochemistry, 2009, 393(1): 145-147.

DOI：10.1016/j.ab.2009.06.013

For many years, chitosan and its derivatives have been considered to be promising biomaterials for tissue engineering and repair. However, information regarding their biological effect on cell phenotype is usually limited to evaluation of cell proliferation and survival, overlooking proteomic and transcriptomic analysis. This is largely related to the lack of efficient and quantitative procedures for protein and nucleic acid purification from cells cultured on, or inside, chitosan scaffold. Here we describe an ultracentrifugation procedure enabling the Simultaneous and quantitative recovery of high quality RNA, DNA and proteins from cells growing in close contact of biomaterial matrices containing chitosan.

• 出版日期2009-10-1