A rapid PCR-RFLP method for the identification of Lophius species

作者：Armani Andrea^*; Castigliego Lorenzo; Tinacci Lara; Gandini Gabriele; Gianfaldoni Daniela; Guidi Alessandra

来源：European Food Research and Technology, 2012, 235(2): 253-263.

DOI：10.1007/s00217-012-1754-3

• PCR-RFLP
• Species identification
• Lophius
• Cytochrome b

The seven Anglerfish species, which belong to the genus Lophius, have a different value on the market, worldwide. If whole fishes can be identified by their morphological characteristics, they become indistinguishable when prepared or processed. In this study, a rapid method based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was developed for the authentication of the seven Lophius species, using a cytochrome b gene fragment of 566 bp. After a genus-specific PCR, a fast digestion with the restriction enzyme BfaI, followed by agarose gel electrophoresis, allowed a clear species identification by producing specific restriction patterns. The total time required was as low as 6 h, DNA extraction included. The method was then used to analyse 48 commercial samples, whose phylogenetic analysis confirmed the PCR-RFLP response at 100 %. Results showed that mislabelling occurs on the market regardless the kind of processing.

• 出版日期2012-8