A NOVEL HPLC-PDA-MS METHOD FOR S-ADENOSYLMETHIONINE AND S-ADENOSYLHOMOCYSTEINE ROUTINE ANALYSIS

作者：Birsan Camelia^*; Litescu Simona C; Radu Gabriel L

来源：Analytical Letters, 2010, 43(5): 793-803.

DOI：10.1080/00032710903486385

• HPLC
• MS
• S-adenosylhomocysteine
• S-adenosylmethionine
• SPE

S-adenosylmethionine and S-adenosylhomocysteine are essential compounds in metabolic pathways; therefore, the development of a suitable method to routinely quantify these compounds is very important. In this paper, a simple and fast method (10 minutes) for simultaneous SAM and SAH quantification in plasma and blood samples is proposed based on the HPLC-PDA-MS technique. A new solid phase extraction procedure for blood samples is reported. The developed method proved to be highly sensitive (1.3 x 10(-9) molL(-1) for SAM and 4.5 x 10(-10) molL(-1) for SAH), repeatable (CV = 1.06% for SAM and CV - 0.92% for SAH), and reproducible (CV - 4.1% for SAM and CV - 3.77% for SAH).

• 出版日期2010
• 单位北京生命科学研究所