Allergic sensitization modifies the pulmonary expression of 5-hydroxytryptamine receptors in guinea pigs

作者：Cordoba Rodriguez Guadalupe; Vargas Mario H; Ruiz Victor; Carbajal Veronica; Campos Bedolla Patricia; Mercadillo Herrera Paulina; Luis Arreola Ramirez Jose; Segura Medina Patricia^*

来源：Respiratory Physiology & Neurobiology, 2016, 223: 9-15.

DOI：10.1016/j.resp.2015.11.018

• Guinea pig
• 5-HT receptors
• Serotonin receptors
• Allergic sensitization
• Asthma model

There is mounting evidence that 5-hydroxytryptamine (5-HT) plays a role in asthma. However, scarce information exists about the pulmonary expression of 5-HT receptors and its modification after allergic sensitization. In the present work, we explored the expression of 5-HT1A, 5-HT2A, 5-HT3, 5-HT4, 5-ht(5a), 5-HT6, and 5-HT7 receptors in lungs from control and sensitized guinea pigs through OCR and Western blot. In control animals, mRNA from all receptors was detectable in lung homogenates, especially from 5-HT2A and 5-HT4 receptors. Sensitized animals had decreased mRNA expression of 5-HT2A and 5-HT4 receptors and increased that of 5-HT7 receptor. In contrast, they had increased protein expression of 5-HT2A receptor in bronchial epithelium and of 5-HT4 receptor in lung parenchyma. The degree of airway response to the allergic challenge was inversely correlated with mRNA expression of the 5-HT1A receptor. In summary, our results showed that major 5-HT receptor subtypes are constitutively expressed in the guinea pig lung, and that allergic sensitization modifies the expression of 5-HT2A, 5-HT4, and 5-HT7 receptors.

• 出版日期2016-3