A hydroquinone biosensor using modified core-shell magnetic nanoparticles supported on carbon paste electrode

作者：Zhang, Yi; Zeng, Guang Ming^*; Tang, Lin; Huang, Dan Lian; Jiang, Xiao Yun; Chen, Yao Ning

来源：Biosensors and Bioelectronics, 2007, 22(9-10): 2121-2126.

DOI：10.1016/j.bios.2006.09.030

• Fe3O4 magnetic nanoparticles
• laccase
• hydroquinone
• biosensor
• compost

A hydroquinone biosensor was developed and used to determine hydroquinone concentration in compost extracts based on the immobilization of laccase on the surface of modified magnetic core-shell (Fe3O4-SiO2) nanoparticles. Laccase was covalently immobilized on the magnetic nanoparticles by glutaraldehyde, which was modified with amino groups on its surface. The obtained magnetic bio-nanoparticles were attached to the surface of carbon paste electrode with the aid of a permanent magnet to determine hydroquinone. A good microenvironment for retaining the bioactivity, of laccase was provided by the immobilization matrix. The linear range for hydroquinone determination was 1 x 10(-7) to 1.375 x 10(-4) M, with a detection limit of 1.5 x 10(-8) M. The current reached 95% of the steady-state current within about 60 s. Hydroquinone concentration in compost extracts was determined by laccase biosensor and HPLC, the results of the two methods were approximately the same.

• 出版日期2007-4-15
• 单位湖南大学