Phenylpropanoids from Lilium Asiatic hybrid flowers and their anti-inflammatory activities

作者：Nhan Nguyen Thi; Song Hae Seong; Oh Eun Ji; Lee Yeong Geun; Ko Jung Hwan; Kwon Jeong Eun; Kang Se Chan; Lee Dae Young; Jung In Ho; Baek Nam In^*

来源：Applied Biological Chemistry, 2017, 60(5): 527-533.

DOI：10.1007/s13765-017-0307-7

• Anti-inflammation
• COX-2
• DPPH
• iNOS
• Lilium Asiatic hybrids
• Phenylpropanoid
• p-p65
• VCAM-1

Three phenylpropanoids were isolated from the flowers of Lilium Asiatic hybrids through repeated silica gel or octadecyl silica gel column chromatographies. The chemical structures were determined to be 1-O-trans-caffeoyl-beta-d-glucopyranoside (1), regaloside A (2), and regaloside B (3), based on spectroscopic data gathered from nuclear magnetic resonance (NMR) spectroscopy, electron ionization mass spectrometry (EI/MS), polarimetry, and infrared spectroscopy (IR) experiments. Compounds 1 and 2 showed significant DPPH radical scavenging activity of 60.1 and 58.0% at 160 ppm, respectively, compared with the 62.0% activity of the positive control, alpha-tocopherol. At a concentration of 50 mu g/mL, compounds 1-3 inhibited the expression of iNOS to 4.1 +/- 0.01, 70.3 +/- 4.07, and 26.2 +/- 0.63, respectively, and decreasing COX-2 expression to 67.8 +/- 4.86, 131.6 +/- 8.19, and 98.9 +/- 4.99. Also, at the same concentration, compounds 1-3 decreased the ratio of p-p65/p-65 to 43.8 +/- 1.67, 40.7 +/- 1.30, and 43.2 +/- 1.60, respectively, and the expression of VCAM-1 to 42.1 +/- 2.31, 48.6 +/- 2.65, and 33.8 +/- 1.74, respectively.

• 出版日期2017-10