Molecular mapping of quantitative trait loci for three kernel-related traits in maize using a double haploid population

作者：Shi, Zi^*; Song, Wei; Xing, Jinfeng; Duan, Minxiao; Wang, Fengge; Tian, Hongli; Xu, Liwen; Wang, Shuaishuai; Su, Aiguo; Li, Chunhui; Zhang, Ruyang; Zhao, Yanxin; Luo, Meijie; Wang, Jidong; Zhao, Jiuran^*

来源：Molecular Breeding, 2017, 37(9): 108.

DOI：10.1007/s11032-017-0706-9

• Kernel-related traits
• QTL mapping
• SNP
• Maize

Kernel size and kernel weight are important factors possibly involved in the determination of grain yield in maize, so identifying the genetic basis of kernel-related traits provides insights into the breeding of high-yield maize varieties. Kernel length (KL), kernel width (KW) and hundred kernel weight (HKW) were evaluated in three various planting conditions for the 240 field-grown double haploid (DH) lines derived from the single-cross hybrid Xianyu335. Variations in KL, KW and HKW were observed among DH lines, and all three traits showed a broad sense heritability of 76%. A total of 964 single nucleotide polymorphisms (SNPs) from the MaizeSNP3072 chip was utilised to create a high-density genetic map of 1546.4 cM and to identify quantitative trait loci (QTLs). Using composite interval mapping, a total of five, seven and five QTLs have been mapped for KL, KW and HKW, respectively. qkl1-2 and qkl4-1 explained 17.8% and 14.2% of the phenotypic variation in KL, respectively, and the other three QTLs contributed 3.2-4.0%. The phenotypic variation explained (PVE) of seven QTLs responsible for KW ranged from 3.3 to 9.5%. Three QTLs for HKW, qhkw1, qhkw5 and qhkw10 each explained more than 10% of the phenotypic variation, and qhkw4 and qhkw9 accounted for 3.0% and 6.0%, respectively. Due to their detection in multiple planting environments, the loci mapped here appear to be potential targets for the improvement of maize grain yield.

• 出版日期2017-9
• 单位北京市农林科学院