Comparison of livestock-associated and health care-associated MRSA-genes, virulence, and resistance

作者：Mutters Nico T^*; Bieber Christian P; Hauck Catherine; Reiner Gerald; Malek Veronika; Frank Uwe

来源：Diagnostic Microbiology and Infectious Disease, 2016, 86(4): 417-421.

DOI：10.1016/j.diagmicrobio.2016.08.016

• MRSA
• CC398
• Livestock
• Infection control
• Genotyping
• Transmission
• Pig farms

Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) may colonize and infect humans with close contact to pigs. We compared phenotypic and genotypic differences in resistance and virulence of LA-MRSA isolates from farms and farmers with hospital-acquired methicillin-resistant S. aureus (HA-MRSA) and assessed carriage rates. Samples from pigs (n = 330), occupationally exposed personnel (n = 63), the farm environment (n = 134), and hospital patients (n = 220) were obtained. Approximately 50% (166/330) of pigs were MRSA positive. All LA-MRSA were resistant to tetracycline, compared to only 8% of HA-MRSA (P < 0.001). In contrast, HA-MRSA isolates showed significantly higher resistance rates to quinolones (81% versus 7%; P < 0.001). All strains isolated from occupationally exposed personnel (61.9%; 39/63) belonged to CC398. HA-MRSA isolates were diversely distributed, with predominance of CC5 (62.7%). Human strains carried significantly more virulence genes than porcine strains, especially exotoxins (P < 0.001) and immune-evasion cluster genes (P < 0.001). There were significant differences in resistance patterns and recognized genotypic virulence loci between LA-MRSA and HA-MRSA.

• 出版日期2016-12