Protein kinase CK2 and new binding partners during spermatogenesis

作者：Mannowetz Nadja; Kartarius Sabine; Wennemuth Gunther; Montenarh Mathias^*

来源：Cellular and Molecular Life Sciences, 2010, 67(22): 3905-3913.

DOI：10.1007/s00018-010-0412-9

• Protein kinase
• Transmission electron microscopy
• Immune histochemistry
• TNP1
• KIF5C
• Spermatogenesis

Protein kinase CK2 is an ubiquitously expressed enzyme that is absolutely necessary for the survival of cells. Besides the holoenzyme consisting of the regulatory beta-subunit and the catalytic alpha- or alpha'-subunit, the subunits exist in separate forms. The subunits bind to a number of other cellular proteins. We show the expression of individual subunits as well as interaction with the transitional nuclear protein TNP1 and with the motor neuron protein KIF5C during spermatogenesis. TNP1 is a newly identified binding partner of the alpha-subunit of CK2. CK2 alpha and KIF5C were found in late spermatogenesis, whereas CK2 beta and TNP1 were found in early spermatogenesis. CK2 alpha, CK2 alpha', TNP1, and KIF5C were detected in the acrosome of spermatozoa, while CK2 beta was detectable in the mid-piece. Combinations of CK2 subunits might determine interactions with other proteins during spermatogenesis. KIF5C as a kinesin motor neuron protein is probably involved in the redistribution of proteins during spermatogenesis.

• 出版日期2010-11