Application of exogenous 24-epibrassinolide enhances proanthocyanidin biosynthesis in Vitis vinifera 'Cabernet Sauvignon' berry skin

作者：Xu, Fan; Gao, Xiang; Xi, Zhu-mei^*; Zhang, Hui; Peng, Xiao-qin; Wang, Zhi-zhen; Wang, Tian-min; Meng, Ying

来源：Plant Growth Regulation, 2015, 75(3): 741-750.

DOI：10.1007/s10725-014-9976-y

• 24-Epibrassinolide
• Grape skin
• Proanthocyanidins biosynthesis
• Flavan-3-ols
• Transcriptional regulation

'Cabernet Sauvignon' (Vitis vinifera L.) grape berries were treated with 0.4 mg/l 24-epibrassinolide applied once at fruit set ('pea-sized berry' phenological stage) (EBR-T1) or the same concentration EBR applied twice at fruit set and 14 days before v,raison (EBR-T2). Berries sprayed with deionized water served as the control. The contents of proanthocyanidins (PAs) in the berry skin and seed, and the expression patterns of structural genes (VvLAR1, VvLAR2, VvANR, and VvANS) and transcription regulator genes (VvMYB5a, VvMYB5b, and VvMYBPA1) of PAs in the berry skin were measured. At berry maturity, total tannins in the skin of EBR-T1- and EBR-T2-treated berries were significant higher than those in control. Monomeric and oligomeric flavan-3-ols showed different levels of promotion by 24-epibrassinolide during berry development. The mRNA levels of the structural genes VvLAR1, VvLAR2, and VvANS and the transcription regulator gene VvMYBPA1 were affected significantly by 24-epibrassinolide treatment, but the effects varied at different stages of berry development. Most of the targets measured showed no significant difference between the EBR-T1 and EBR-T2 treatments. Thus, in general, application of 24-epibrassinolide enhanced PAs biosynthesis in the berry skin.

• 出版日期2015-4
• 单位西北农林科技大学