Measurements of transposition frequency of insertion sequence IS1 by GFP hop-on assay

作者：Saito Takashi; Chibazakura Taku; Takahashi Kiwamu; Yoshikawa Hirofumi; Sekine Yasuhiko^*

来源：Journal of General and Applied Microbiology, 2010, 56(3): 187-192.

• Escherichia coli
• fluorescence-activated cell sorting (FACS)
• green fluorescent protein (GFP)
• H-NS
• insertion sequence
• IS1
• transposition

Transposons play a significant role in the evolution of bacterial genomes. Quantifying frequency of transpositional events caused by a transposon will facilitate understanding its role. Here, we report successful measurement of the frequency of IS1 transposition using "GFP hop-on assay" in which transposition-dependent GFP expression is monitored by FACS. This assay allows easy assessment of IS transposition into the chromosomal DNA on a single-cell scale; this is an advantage over other conventional methods to measure transposition frequency.

• 出版日期2010-6