Determination of Sulfite in Beer Based on Fluorescent Derivatives and Liquid Chromatographic Separation

作者：Abrahamsson Victor; Hoff Signe; Nielsen Nikoline J; Lund Marianne N; Andersen Mogens L^*

来源：Journal of the American Society of Brewing Chemists, 2012, 70(4): 296-302.

DOI：10.1094/ASBCJ-2012-1012-01

• Analysis
• Beer
• Matrix match
• Sulfite
• ThioGlo 1

J. Am. Soc. Brew. Chem. 70(4):296-302, 2012 A method was developed for quantification of sulfite in beer based on derivatization with the maleimide-derived probe ThioGlo I followed by separation of fluorescent adducts by reversed-phase high-performance liquid chromatography and fluorescence detection. Sulfite gave two ThioGlo 1 derivatives and it was shown by mass spectrometry that both had identical mass spectra. Matrix effects were observed when constructing sulfite standard curves in different beers and, therefore, use of a matrix-matched calibration curve is proposed. ThioGlo I was found to generate fluorescent adducts with both bound and free sulfite, providing a quantification of the total sulfite content in beer. The limit of quantification of sulfite was 0.6 mg/L and the method can be used for quantification of sulfite in highly colored beers.

• 出版日期2012