Activation of beta-Adrenoceptors by Dobutamine May Induce a Higher Expression of Peroxisome Proliferator-Activated Receptors delta (PPAR delta) in Neonatal Rat Cardiomyocytes

作者：Chou, Ming Ting; Lo, Shih Hsiang; Cheng, Kai Chun; Li, Yin Xiao; Chen, Li Jen; Cheng, Juei Tang^*

来源：The Scientific World Journal, 2012, 2012: 248320.

DOI：10.1100/2012/248320

Recent evidence showed the role of peroxisome proliferator-activated receptors (PPARs) in cardiac function. Cardiac contraction induced by various agents is critical in restoring the activity of peroxisome proliferator-activated receptors delta (PPAR delta) in cardiac myopathy. Because dobutamine is an agent widely used to treat heart failure in emergency setting, this study is aimed to investigate the change of PPAR delta in response to dobutamine. Neonatal rat cardiomyocytes were used to examine the effects of dobutamine on PPAR delta expression levels and cardiac troponin I (cTnI) phosphorylation via Western blotting analysis. We show that treatment with dobutamine increased PPAR delta expression and cTnI phosphorylation in a time-and dose-dependent manner in neonatal rat cardiomyocytes. These increases were blocked by the antagonist of beta 1-adrenoceptors. Also, the action of dobutamine was related to the increase of calcium ions and diminished by chelating intracellular calcium. Additionally, dobutamine-induced action was reduced by the inhibition of downstream messengers involved in this calcium-related pathway. Moreover, deletion of PPAR delta using siRNA generated the reduction of cTnI phosphorylation in cardiomyocytes treated with dobutamine. Thus, we concluded that PPAR delta is increased by dobutamine in cardiac cells.

• 出版日期2012
• 单位河北医科大学