A novel fluorescence in situ hybridization test for rapid pathogen identification in positive blood cultures

作者：Makristathis A^*; Riss S; Hirschl A M

来源：Clinical Microbiology and Infection, 2014, 20(10): O760-O763.

DOI：10.1111/1469-0691.12561

• Blood culture
• fluorescence insitu hybridization
• identification
• pathogen

A novel molecular beacon-based fluorescence insitu hybridization (FISH) test allowing for the identification of a wide range of bacterial pathogens directly in positive blood cultures (BCs) was evaluated with positive BCs of 152 patients. Depending on the Gram stain, either a Gram-negative or a Gram-positive panel was used. The time to result was 30min, and the hands-on time was only 10min. Seven percent of the cultured microorganisms were not included in the FISH panels; the identification rate of those included was 95.2%. Overall, the FISH test enabled accurate pathogen identification in 88.2% of all cases analysed.

• 出版日期2014-10