Analyzing metabolomics-based challenge tests

作者：Vis Daniel J^*; Westerhuis Johan A; Jacobs Doris M; van Duynhoven John P M; Wopereis Suzan; van Ommen Ben; Hendriks Margriet M W B; Smilde Age K

来源：Metabolomics, 2015, 11(1): 50-63.

DOI：10.1007/s11306-014-0673-7

• Challenge tests
• Perturbation
• Statistical analysis
• Review
• Redefining health

Challenge tests are used to assess the resilience of human beings to perturbations by analyzing responses to detect functional abnormalities. Well known examples are allergy tests and glucose tolerance tests. Increasingly, metabolomics analysis of blood or serum samples is used to analyze the biological response of the individual to these challenges. The information content of such metabolomics challenge test data involves both the disturbance and restoration of homeostasis on a metabolic level and is thus inherently different from the analysis of steady state data. It opens doors to study the variation of resilience between individuals beyond the classical biomarkers; preferably in terms of underlying biological processes. We review challenge tests in which metabolomics was used to analyze the biological response. Specifically, we describe strategies to perform statistical analyses on the responses and we will show some examples of these strategies applied to a postprandial challenge that was used to study a diet with anti-inflammatory properties. Finally we discuss open issues and give recommendation for further research.

• 出版日期2015-2