Actinokineospora mzabensis sp nov., a novel actinomycete isolated from Saharan soil

作者：Aouiche Adel; Bouras Noureddine; Mokrane Salim; Zitouni Abdelghani; Schumann Peter; Sproeer Cathrin; Sabaou Nasserdine^*; Klenk Hans Peter

来源：Antonie van Leeuwenhoek, 2015, 107(1): 291-296.

DOI：10.1007/s10482-014-0328-8

• Actinokineospora
• Actinokineospora mzabensis sp nov.
• Saharan soil
• Polyphasic taxonomy

A novel actinomycete strain, designated PAL84, was isolated from a Saharan soil sample collected from B,ni-Isguen, Gharda < a (South of Algeria). This strain was studied for its taxonomic position using a polyphasic approach and was identified as a member of the genus Actinokineospora. Phylogenetic analysis showed that strain PAL84 had 16S rRNA gene sequence similarities with members of the genus Actinokineospora ranging from 96.2 % (Actinokineospora inagensis DSM 44258(T)) to 97.8 % (Actinokineospora baliensis NBRC 104211(T)). The strain was observed to produce pinkish-purple aerial mycelium and purplish red substrate mycelium, which fragmented readily into chains of non-motile elements. The optimum growth temperature and pH were found to be 25-30 A degrees C and 5.0-7.0, respectively. The cell-wall hydrolysate of strain PAL84 was found to contain meso-diaminopimelic acid and the diagnostic whole-cell sugars were identified as arabinose and galactose. The predominant menaquinone was identified as MK-9 (H-4). The major fatty acids were found to be iso-C-16:0, iso-C-15:0, iso-C-16:1 H and iso-C-16:0 2OH. The diagnostic phospholipid detected was phosphatidylethanolamine. The genotypic and phenotypic data show that the strain represents a novel species of the genus Actinokineospora, for which the name Actinokineospora mzabensis sp. nov. is proposed, with the type strain PAL84(T) (=DSM 45961(T) = CECT 8578(T)).

• 出版日期2015-1