A novel spermatogenesis-related factor-1 gene expressed in maturing rat testis

作者：Yamano Y^*; Ohyama K; Sano T; Ohta M; Shimada A; Hirakawa Y; Sugimoto M; Morishima I

来源：Biochemical and Biophysical Research Communications, 2001, 289(4): 888-893.

DOI：10.1006/bbrc.2001.6048

• spermatogenesis
• spermatogonia
• spermatocyte
• rat testis
• kinesin-related protein
• meiosis
• differential display

A rat gene with testis-specific expression coinciding with spermatogenesis was cloned by differential display. This spermatogenesis-related factor-1 (SRF-1) gene was not expressed in other organs. Testicular expression was detected from 5 weeks of age and increased up to 15 weeks; this level of expression was maintained for 63 weeks. The 750-bp cloned gene was coded for an open reading frame of 202 amino acids. According to in situ hybridization at 7 weeks, this gene was expressed mainly in spermatocyte. The gene product may function as a molecular motor in meiosis, as the deduced amino acid sequence showed partial homology with kinesin-related proteins. The action of this gene and its product with respect to division of reproductive cells requires further investigation.

• 出版日期2001-12-14