Mining human antibody repertoires

作者：Beerli Roger R^*; Rader Christoph

来源：mAbs, 2010, 2(4): 365-378.

DOI：10.4161/mabs.12187

• human monoclonal antibodies
• B cells
• hybridoma technology
• display technologies
• antibody libraries
• antibody engineering

Human monoclonal antibodies (mAbs) have become drugs of choice for the management of an increasing number of human diseases. Human antibody repertoires provide a rich source for human mAbs. Here we review the characteristics of natural and non-natural human antibody repertoires and their mining with non-combinatorial and combinatorial strategies. In particular, we discuss the selection of human mAbs from naive, immune, transgenic and synthetic human antibody repertoires using methods based on hybridoma technology, clonal expansion of peripheral B cells, single-cell PCR, phage display, yeast display and mammalian cell display. Our reliance on different strategies is shifting as we gain experience and refine methods to the efficient generation of human mAbs with superior pharmacokinetic and pharmacodynamic properties.

• 出版日期2010-8