Design of a system to measure light scattering from individual cells excited by an acoustic wave

作者:Georgescu Ramona; Khismatullin Damir; Holt R Glynn; Castagner Jean Luc; A'amar Ousama; Bigio Irving J*
来源:Optics Express, 2008, 16(6): 3496-3503.
DOI:10.1364/OE.16.003496

摘要

A system to measure light scattering from individual cells excited by an acoustic wave was designed, and tests were performed on live Jurkat cells. Cells passing in a laminar stream within a water bath were excited by a focused ultrasound pulse, while the scattered light from a laser beam was monitored at various scattering angles. The cells were modeled as viscoelastic liquid drops, which return to equilibrium via shape oscillations after an acoustically-induced deformation. The Fast Fourier Transform of the scattered light signal was used to extract information about the highly-damped resonant frequencies of the cells, and the detected frequencies are consistent with theoretical predictions.

  • 出版日期2008-3-17